Journal details
Comparative histomorphometrical study on testis of indigenous Bull and Black Bengal Buck
Published Date: 30 Dec 2016
Keywords: : Histomorphology, Puberty, Seminiferous Tubules, Spermatogenic cells, Stromal cells
Md. Royhan Gofur1*, Md. Najmul Hassan Parvez2 and Sanjoy Kumar Kabiraj3 1Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh 2Department of Anatomy and Histology, Hajee Mohammad Danesh Science and Technology University, Bangaldesh 3Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh
Abastract:
Context: A comprehensive knowledge of normal histomorphology of testis is important for detecting testicular abnormalities. Objectives: The present study was designed to study and compare the histomorphometrical features of testis of indigenous bulls (Bos indicus) and Black Bengal bucks (Capra hircus) at the age just after puberty. Materials and Methods: Histomorphometry of the testis of indigenous bulls (n=5) and Black Bengal bucks (n=5) at the age just after puberty was studied and compared using routine Hematoxylin and Eosin (H&E) staining technique. Results: The testis of both species was surrounded by visceral layer of tunica vaginalis (consisted of mesothelium and connective tissue) and tunica albugenia. The seminiferous tubules were tortuous, two ended loops and varying in appearance and the wall of tubules consisted of lamina propria, basement membrane and a lining of complex stratified epithelium consisted of Sertoli cells and spermatogenic cells. The sertoli cells are irregularly columnar cells, extended from basal lamina to lumen of tubules and the spermatogenic cells situated between the Sertoli cells in an orderly manner with four to eight layers (in bull) or four to six layers (in buck) occupying the space between the basal lamina and the lumen of the tubules. There was presence of both spermatid and spermatozoa in the lumen of some seminiferous tubules of testes of both species. The interstitial tissues located between the seminiferous tubules, consisted of connective tissue network, blood and lymph vessels with stromal cells. The stromal cells including the Leydig cells were present as single or groups within intertubular spaces. The thickness of tunica albuginea, cross sectional length and breadth of the seminiferous tubules and the number of stromal cells were significantly (p<0.01) more in the testis of bulls than bucks. There was a significant (p<0.01) correlation (r=1.0) between the length and breadth of seminiferous tubules of testis of both species of animals. In between left and right testes, the thickness of tunica albuginea and cross sectional length and breadth of the seminiferous tubules were higher in the left testis but the number of stromal cells was higher in right testis in both indigenous bulls and Black Bengal bucks. Conclusions: The histological architecture of bull and buck was same with some histomorphometrical differences. The thickness of tunica albuginea, cross sectional length and breadth of the seminiferous tubules and the number of stromal cells were significantly higher in the testis of bulls than bucks.