Journal details
Isolation, identification and antibiogram study of Escherichia coli from the cases of mastitis

Published Date: 13 Nov 2019

Keywords: Mastitis, bacterial pathogen, dairy farm, antibiogram, drug of choice


Md. Majadur Rahman, 1Md. Fakhruzzaman2, Md. Mostafizer Rahman2,
Rashida Khaton3, Shah Md. Iqbal4, Shyla Sharmin and Md. Hemayatul Islam3

1Bangladesh University of Professional, Mirpur Cantonment, Dhaka, Bangladesh; 1 & 2Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200; 3Department of Veterinary and Animal Sciences, University of Rajshahi, Bangladesh; 4Department of Livestock Services, Ministry of Fishery and Livestock, Bangladesh




Context: Mastitis is the devastating diseases among the dairy farms in Bangladesh.

Objectives: To isolate, identify and antibiogram study of Escherichia coli from the cases of mastitis among suspected dairy farm.

Materials and methods:The study was conducted to isolate, identify and antibiogram study of significant bacterial pathogens causing mastitis in dairy cow. The study was conducted on 50 suspected dairy cows from 5 commercial dairy farms and Upazilla veterinary hospitals during the period from March, 2011 to August, 2011 of Gaibandha district under the Department of Microbiology Hajee Mohammed Danesh Science and Technology University, Dinajpur, Bangladesh.

Results: Result found on milk samples from different sources were 30% in positive for Escherichia coli, 20% were found to be positive for Staphylococcus species. 20% for Streptococcus species, 14% were Corynebacterium species and16% were undefined species of mixed bacteria. Results of the antibiograms among various isolates of Escherichia coli were found to be highly sensitive (+++) to Chloramphenicol (70%), Kanamycin (70%) and Nitrofurantoin (90%).

Conclusion: Among the isolated bacterial pathogen Escherichia coli plays significant role (30%) on mastitis infection and it is highly sensitive to Nitrofurantoin, Chloramphenicol and Kanamycin. As Nitrofurantoin has the huge public health hazard, so drug of choice can be the Chloramphenicol and Kanamycin against Escherichia coli.