Journal details
Biometrical and histological studies on the epididymis of ram in chars of Pabna region
Published Date: 30 Dec 2015
Keywords: Epididymis, Biometrical, Ram, Histology.
Md. Sarif Ahmmad*, Md. Najmul Hassan Parvez, Khadija Al Ferdous and Md. Sadequl Islam
Department of Anatomy and Histology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Abastract:
Objectives: The objectives of this research were to study gross anatomy including length, weight, size, shape, volume and circumference; and the microscopic structures of the epididymis with the special emphasis on the biometrical comparison between left and right epididymis.
Meterials and Methods: For this purpose, epididymes of six adult rams were used and biometrical values were recorded. For microscopic studies, tissue sample were excised from the epiddidymis, fixed in 10% formalin solution and embedded in paraffin. Cross sections (6 µm thickness) were stained with hematoxylin and eosin and evaluated the histological structures of the epididymal caput, corpus and cauda. Statistical analysis of the data was done by paired sample t-test using SPSS 16.0 programme.
Result: The mean values of length, weight, volume and circumference along with of the left epididymis were 19.58±0.18 cm, 11.65±0.17 gm, 13.65±0.01 ml and 14.27±0.11 cm and for right epididymis 19.53±0.15 cm, 11.41±0.13 gm, 13.65±0.00 ml and 14.22±0.07 cm, respectively. The mean value of caput weight, corpus weight and cauda weight for left epididymis were 4.05±0.06 gm, 1.61±0.02 gm and 5.99±0.09 gm, for right epididymis 3.97±0.05 gm, 1.58±0.02 gm and 5.87±0.07 gm respectively. The mean value of caput length, corpus length and cauda length for left epididymis were 9.35±0.171 cm, 8.450±0.096 cm and 1.783±0.069 cm, for right epididymis 9.35±0.171 cm, 8.417±0.09 cm and 1.767±0.047 cm, respectively. The ductus epididymis is lined with a pseudostratified stereociliated columnar epithelium surrounded by a small amount of loose connective tissue and circular smooth muscle fibers. Few sperm were found in the initial segment, but a large mass of sperm aggregates in the cauda.
Conclusion: Grossly the length, weight, volume and circumference of the left epididymis is higher than that of the right and histologically the ductus epididymis is lined with a pseudostratified stereociliated columnar epithelium surrounded by a small amount of loose connective tissue and circular smooth muscle fibers and a large mass of sperm aggregates in the cauda of the epididymis.