Journal details
Effects of high level of fungal phytase on newcastle disease vaccination in broiler chickens
Published Date: 30 Dec 2015
Keywords: Broiler chicken, Fungal phytase, ND vaccination, Immune responses, Body weight, Hemato- biochemical constituents.
Rakibul Islam1*, Aini Ideris1, Azhar Kasim2, Abdul Rahman Omar1, Anis Shobirin Meor Hussin3, Farhana Yasmin1 and Yeasmin Akter2
1Department of Pathology and Microbiology, Universiti Putra Malaysia, 2Department of Animal Science, Universiti Putra Malaysia, 3Department of Food Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Abastract:
Objectives: An experiment was carried out in broiler chicken fed two doses of commercially produced fungal phytase (Natuphos®) addition to observe their growth performance as health status, and to investigate the changes of hemato-biochemical constituents and immune response.
Materials and Methods: Seventy two male broiler chickens (Cobb) at one-day old were allocated to two treatment (T) groups with six cages comprising three replicates, each cage containing twelve birds. Experimental formulating diets arranged with two levels of 0 and 1500 (FTU/kg-1 of diet) Natuphos® as considered as T0 and T1, respectively. At seven days intervals, two birds were randomly selected from each treatment, and were weighted for assessing the growth performance and slaughtered for collection of blood and jejunal fluid throughout the experimental period to quantify specific antibody, IgM and IgG, as well as IgA, respectively. At the age of six week, blood was furthermore collected for determining the complete hemogram and blood biochemical constituents. Specific antibodies against Newcastle disease vaccine (NDV) was detected by ELISA.
Results: Data showed that the inclusion of Natuphos® at the dose rate of 1500 FTU kg-1 of diet, the significant (P<0.05) differences were observed starting from the age of second week and continued until six week. The main effects data on growth performance revealed that average body weight gains of phytase treated birds were increased significantly (P<0.05) at all ages. In terms of humoral immune response, data showed that a specific antibody production against NDV was not improved by Natuphos® addition in a low P diet. However, although non-specific blood IgM and IgG levels were not enhanced, the secretory jejunal IgA content in enzyme treated group was increased throughout the experiments. Conversely, no significant, sequential, and consistent changed effects were observed on complete hemogram and blood biochemical constituents in broiler chickens by Natuphos® treatments.
Conclusion: The results obtained in this study decided that high level of fungal phytase (Natuphos®) addition had no side effect on hematological parameters and biochemical constituents at blood level. Rather it enhances the growth performance and mucosal secretory IgA concentration on ND vaccination.